Metabolic Biodegradation Pathway of Fluoranthene by Indigenous Trichoderma lixii and Talaromyces pinophilus spp.

Two indigenous ascomycetes fungi, Trichoderma lixii strain FLU1 (TlFLU1) and Talaromyces pinophilus FLU12 (TpFLU12), were isolated from benzo(b)fluoranthene-enriched activated sludge tested for bio-catalytically degrade fluoranthene as a sole carbon source. TlFLU1 TpFLU12 degraded 98 99% of 400 mg/L after 16 12 d incubation period, respectively. Degradation correlated with the upregulation expression ligninolytic enzymes. The GC-MS FTIR analysis degradation products suggest that is initiated at C1-C2 position compound ring via oxygenation cleavage to form 9-oxo-9H-fluorene-1-carboxylic acid before undergoing yield fluorenone, which then proceeds through ß-Ketoadipate pathway benzene-1,2,3-tricarboxylic acid. rate better fitted in first-order zero-order kinetic model TpFLU12, metabolites media are shown be toxic Vibryo parahaemolyticus 6 h exposure effective concentration (EC50) toxicity unit (TU) values 14.25 7.018%, respectively, while also being observed non-toxic an EC50 TU 197.1 0.507%, Results this study show efficient metabolism into innocuous state by TpFLU12.